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Comparative Analysis of a Series of pH‐Responsive Sulphonated Europium Complexes for Bioassays Monitoring Acidification

Fradgley, Jack D.; Starck, Matthieu; Lamarque, Laurent; Parker, David

Comparative Analysis of a Series of pH‐Responsive Sulphonated Europium Complexes for Bioassays Monitoring Acidification Thumbnail


Authors

Jack D. Fradgley

Matthieu Starck

Laurent Lamarque



Abstract

The synthesis and photophysical evaluation of a series of hydrophilic sulphonated and pH responsive luminescent europium(III) probes are reported. The Eu emission intensities and lifetimes increase very significantly on lowering pH, leading to a ‘switching on’ of Eu luminescence in more acidic media. By varying the nature of the substituents at an integral aniline nitrogen atom located in the sensitising chromophore, the pKa value of the complex can be tuned to between 4.3 and 6.3 for a set of five Eu(III) complexes. These systems are designed to allow conjugation to targeting vectors permitting, for example, the monitoring of receptor internalisation in cells. This approach can be used to tag proteins selectively and allows the monitoring of uptake into acidic organelles, enabling the creation of time-resolved internalisation assays to follow pH change in real time, both in vitro and in cellulo.

Citation

Fradgley, J. D., Starck, M., Lamarque, L., & Parker, D. (2022). Comparative Analysis of a Series of pH‐Responsive Sulphonated Europium Complexes for Bioassays Monitoring Acidification. European Journal of Inorganic Chemistry, 2022(32), Article e202200426. https://doi.org/10.1002/ejic.202200426

Journal Article Type Article
Acceptance Date Jul 22, 2022
Online Publication Date Aug 12, 2022
Publication Date Nov 17, 2022
Deposit Date Sep 5, 2022
Publicly Available Date Mar 28, 2024
Journal European Journal of Inorganic Chemistry
Print ISSN 1434-1948
Electronic ISSN 1099-0682
Publisher Wiley-VCH Verlag
Peer Reviewed Peer Reviewed
Volume 2022
Issue 32
Article Number e202200426
DOI https://doi.org/10.1002/ejic.202200426

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Publisher Licence URL
http://creativecommons.org/licenses/by/4.0/

Copyright Statement
© 2022 The Authors. European Journal of Inorganic Chemistry published by Wiley-VCH GmbH

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.




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