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Design considerations of benchtop fluid flow bioreactors for bio-engineered tissue equivalents in vitro

Hoyle, H.W.; Stenger, C.M.L.; Przyborski, S.A.

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Authors

C.M.L. Stenger



Abstract

One of the major aims of bio-engineering tissue equivalents in vitro is to create physiologically relevant culture conditions to accurately recreate the cellular microenvironment. This often includes incorporation of factors such as the extracellular matrix, co-culture of multiple cell types and three-dimensional culture techniques. These advanced techniques can recapitulate some of the properties of tissue in vivo, however fluid flow is a key aspect that is often absent. Fluid flow can be introduced into cell and tissue culture using bioreactors, which are becoming increasingly common as we seek to produce increasingly accurate tissue models. Bespoke technology is continuously being developed to tailor systems for specific applications and to allow compatibility with a range of culture techniques. For effective perfusion of a tissue culture many parameters can be controlled, ranging from impacts of the fluid flow such as increased shear stress and mass transport, to potentially unwanted side effects such as temperature fluctuations. A thorough understanding of these properties and their implications on the culture model can aid with a more accurate interpretation of results. Improved and more complete characterisation of bioreactor properties will also lead to greater accuracy when reporting culture conditions in protocols, aiding experimental reproducibility, and allowing more precise comparison of results between different systems. In this review we provide an analysis of the different factors involved in the development of benchtop flow bioreactors and their potential biological impacts across a range of applications.

Citation

Hoyle, H., Stenger, C., & Przyborski, S. (2022). Design considerations of benchtop fluid flow bioreactors for bio-engineered tissue equivalents in vitro. Biomaterials and biosystems, 8, Article 100063. https://doi.org/10.1016/j.bbiosy.2022.100063

Journal Article Type Article
Acceptance Date Aug 30, 2022
Online Publication Date Aug 31, 2022
Publication Date 2022
Deposit Date Jan 23, 2023
Publicly Available Date Mar 29, 2024
Journal Biomaterials and Biosystems
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 8
Article Number 100063
DOI https://doi.org/10.1016/j.bbiosy.2022.100063

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