Identification of potentially zoonotic parasites in captive orangutans and semi-captive mandrills: phylogeny and morphological comparison

Nosková, Eva; Modrý, David; Baláž, Vojtech; Červená, Barbora; Jirků-Pomajbíková, Kateřina; Zechmeisterová, Kristína; Leowski, Clotilde; Petrželková, Klára J.; Pšenková, Ilona; Vodička, Roman; Kessler, Sharon E.; Ngoubangoye, Barthélémy; Setchell, Joanna M.; Pafčo, Barbora

doi:10.1002/ajp.23475

Identification of potentially zoonotic parasites in captive orangutans and semi-captive mandrills: phylogeny and morphological comparison

Nosková, Eva; Modrý, David; Baláž, Vojtech; Červená, Barbora; Jirků-Pomajbíková, Kateřina; Zechmeisterová, Kristína; Leowski, Clotilde; Petrželková, Klára J.; Pšenková, Ilona; Vodička, Roman; Kessler, Sharon E.; Ngoubangoye, Barthélémy; Setchell, Joanna M.; Pafčo, Barbora

Authors

Eva Nosková

David Modrý

Vojtech Baláž

Barbora Červená

Kateřina Jirků-Pomajbíková

Kristína Zechmeisterová

Clotilde Leowski

Klára J. Petrželková

Ilona Pšenková

Roman Vodička

Sharon E. Kessler

Barthélémy Ngoubangoye

Professor Jo Setchell joanna.setchell@durham.ac.uk
Professor

Barbora Pafčo

Abstract

Cysts and trophozoites of vestibuliferid ciliates and larvae of Strongyloides were found in fecal samples from captive orangutans Pongo pygmaeus and P. abelii from Czech and Slovak zoological gardens. As comparative material, ciliates from semi-captive mandrills Mandrillus sphinx from Gabon were included in the study. Phylogenetic analysis of the detected vestibuliferid ciliates using ITS1-5.8s-rRNA-ITS2 and partial 18S rDNA revealed that the ciliates from orangutans are conspecific with Balantioides coli lineage A, while the ciliates from mandrills clustered with Buxtonella-like ciliates from other primates. Morphological examination of the cysts and trophozoites using light microscopy did not reveal differences robust enough to identify the genera of the ciliates. Phylogenetic analysis of detected L1 larvae of Strongyloides using partial cox1 revealed Strongyloides stercoralis clustering within the cox1 lineage A infecting dogs, humas and other primates. The sequences of 18S rDNA support these results. As both B. coli and S. stercoralis are zoonotic parasites and the conditions in captive and semi-captive settings may facilitate transmission to humans, prophylactic measures should reflect the findings.

Citation

Nosková, E., Modrý, D., Baláž, V., Červená, B., Jirků-Pomajbíková, K., Zechmeisterová, K., …Pafčo, B. (2023). Identification of potentially zoonotic parasites in captive orangutans and semi-captive mandrills: phylogeny and morphological comparison. American Journal of Primatology, 85(4), Article e23475. https://doi.org/10.1002/ajp.23475

Journal Article Type	Article
Acceptance Date	Jan 23, 2023
Online Publication Date	Feb 13, 2023
Publication Date	2023-04
Deposit Date	Jan 25, 2023
Publicly Available Date	Jul 19, 2023
Journal	American Journal of Primatology
Print ISSN	0275-2565
Electronic ISSN	1098-2345
Publisher	Wiley
Peer Reviewed	Peer Reviewed
Volume	85
Issue	4
Article Number	e23475
DOI	https://doi.org/10.1002/ajp.23475

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© 2023 The Authors. American Journal of Primatology published by Wiley Periodicals LLC.

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