Julia Bruggisser
Cryo‐EM structure of the octameric pore of Clostridium perfringens β‐toxin
Bruggisser, Julia; Iacovache, Ioan; Musson, Samuel C; Degiacomi, Matteo T; Posthaus, Horst; Zuber, Benoît
Authors
Ioan Iacovache
Sam Musson samuel.musson@durham.ac.uk
PGR Student Doctor of Philosophy
Matteo T Degiacomi
Horst Posthaus
Benoît Zuber
Abstract
Clostridium perfringens is one of the most widely distributed and successful pathogens producing an impressive arsenal of toxins. One of the most potent toxins produced is the C. perfringens β-toxin (CPB). This toxin is the main virulence factor of type C strains. We describe the cryo-electron microscopy (EM) structure of CPB oligomer. We show that CPB forms homo-octameric pores like the hetero-oligomeric pores of the bi-component leukocidins, with important differences in the receptor binding region and the N-terminal latch domain. Intriguingly, the octameric CPB pore complex contains a second 16-stranded β-barrel protrusion atop of the cap domain that is formed by the N-termini of the eight protomers. We propose that CPB, together with the newly identified Epx toxins, is a member a new subclass of the hemolysin-like family. In addition, we show that the β-barrel protrusion domain can be modified without affecting the pore-forming ability, thus making the pore particularly attractive for macromolecule sensing and nanotechnology. The cryo-EM structure of the octameric pore of CPB will facilitate future developments in both nanotechnology and basic research.
Citation
Bruggisser, J., Iacovache, I., Musson, S. C., Degiacomi, M. T., Posthaus, H., & Zuber, B. (2022). Cryo‐EM structure of the octameric pore of Clostridium perfringens β‐toxin. EMBO Reports, 23(12), Article e54856. https://doi.org/10.15252/embr.202254856
Journal Article Type | Article |
---|---|
Acceptance Date | Sep 14, 2022 |
Online Publication Date | Dec 6, 2022 |
Publication Date | Dec 6, 2022 |
Deposit Date | Apr 5, 2023 |
Publicly Available Date | Mar 28, 2024 |
Journal | EMBO reports |
Print ISSN | 1469-221X |
Electronic ISSN | 1469-3178 |
Publisher | Wiley |
Peer Reviewed | Peer Reviewed |
Volume | 23 |
Issue | 12 |
Article Number | e54856 |
DOI | https://doi.org/10.15252/embr.202254856 |
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Copyright Statement
Open Access. This article is distributed under the terms of the Creative Commons Attribution License (CC-BY 4.0), which permits any use, distribution and reproduction in any medium, provided the original author(s) and source are credited.
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