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Down-regulation of vimentin expression inhibits carcinoma cell migration and adhesion

McInroy, Lorna; Maatta, Arto

Authors

Lorna McInroy



Abstract

Vimentin is a type III Intermediate filament protein that is expressed frequently in epithelial carcinomas correlating with invasiveness and poor prognosis. We have analysed migration and adhesion to collagenous matrix of a panel of carcinoma cell lines. In vitro invasiveness was highest in vimentin-positive SW480 colon cancer and MDA-MB-231 breast cancer cells and the role of vimentin in these cell lines was investigated by RNA interference. Down-regulation of vimentin expression resulted in impaired migration in both scratch-wound experiments and in invasion assays through cell culture inserts coated with collagen gel. Compromised migration was observed in both cell lines, whereas cell attachment assays revealed impaired adhesion to fibrillar collagen in MDA-MB-231 cells while the adhesion of vimentin-ablated SW480 cells, that express both vimentin and keratin intermediate filaments was not affected. In conclusion, ablation of vimentin expression inhibits migration and invasion of colon and breast cancer cell lines.

Citation

McInroy, L., & Maatta, A. (2007). Down-regulation of vimentin expression inhibits carcinoma cell migration and adhesion. Biochemical and Biophysical Research Communications, 360(1), 109-114. https://doi.org/10.1016/j.bbrc.2007.06.036

Journal Article Type Article
Publication Date Aug 1, 2007
Deposit Date Jul 9, 2007
Journal Biochemical and Biophysical Research Communications
Print ISSN 0006-291X
Electronic ISSN 1090-2104
Publisher Academic Press
Peer Reviewed Peer Reviewed
Volume 360
Issue 1
Pages 109-114
DOI https://doi.org/10.1016/j.bbrc.2007.06.036
Keywords Intermediate filaments, Vimentin, RNA interference, Invasion, Cell migration, Cell adhesion.