Tholozan, F. M. and Gribbon, C. and Li, Z. and Goldberg, M. W. and Prescott, A. R. and McKie, N. and Quinlan, R. A. (2007) 'FGF-2 release from the lens capsule by MMP-2 maintains lens epithelial cell viability.', Molecular biology of the cell., 18 (11). pp. 4222-4231.
The lens is an avascular tissue, separated from the aqueous and vitreous humors by its own extracellular matrix, the lens capsule. Here we demonstrate that the lens capsule is a source of essential survival factors for lens epithelial cells. Primary and immortalized lens epithelial cells survive in low levels of serum and are resistant to staurosporine-induced apoptosis when they remain in contact with the lens capsule. Physical contact with the capsule is required for maximal resistance to stress. The lens capsule is also a source of soluble factors including fibroblast growth factor 2 (FGF-2) and perlecan, an extracellular matrix component that enhances FGF-2 activity. Matrix metalloproteinase 2 (MMP-2) inhibition as well as MMP-2 pretreatment of lens capsules greatly reduced the protective effect of the lens capsule, although this could be largely reversed by the addition of either conditioned medium or recombinant FGF-2. These data suggest that FGF-2 release from the lens capsule by MMP-2 is essential to lens epithelial cell viability and survival.
|Full text:||(VoR) Version of Record|
Download PDF (476Kb)
|Publisher Web site:||http://dx.doi.org/10.1091/mbc.E06-05-0416|
|Publisher statement:||© 2007 by The American Society for Cell Biology|
|Date accepted:||No date available|
|Date deposited:||01 October 2008|
|Date of first online publication:||November 2007|
|Date first made open access:||No date available|
Save or Share this output
|Look up in GoogleScholar|