T. Libotte
Lamin A/C-dependent localization of Nesprin-2, a giant scaffolder at the nuclear envelope
Libotte, T.; Zaim, H.; Abraham, S.; Padmakumar, V.C.; Schneider, M.; Lu, W.S.; Munck, M.; Hutchison, C.; Wehnert, M.; Fahrenkrog, B.; Sauder, U.; Aebi, U.; Noegel, A.A.; Karakesisoglou, I.
Authors
H. Zaim
S. Abraham
V.C. Padmakumar
M. Schneider
W.S. Lu
M. Munck
C. Hutchison
M. Wehnert
B. Fahrenkrog
U. Sauder
U. Aebi
A.A. Noegel
Dr Iakowos Karakesisoglou iakowos.karakesisoglou@durham.ac.uk
Associate Professor
Abstract
The vertebrate proteins Nesprin-1 and Nesprin-2 (also referred to as Enaptin and NUANCE) together with ANC-1 of Caenorhabditis elegans and MSP-300 of Drosophila melanogaster belong to a novel family of -actinin type actin-binding proteins residing at the nuclear membrane. Using biochemical techniques, we demonstrate that Nesprin-2 binds directly to emerin and the C-terminal common region of lamin A/C. Selective disruption of the lamin A/C network in COS7 cells, using a dominant negative lamin B mutant, resulted in the redistribution of Nesprin-2. Furthermore, using lamin A/C knockout fibroblasts we show that lamin A/C is necessary for the nuclear envelope localization of Nesprin-2. In normal skin where lamin A/C is differentially expressed, strong Nesprin-2 expression was found in all epidermal layers, including the basal layer where only lamin C is present. This indicates that lamin C is sufficient for proper Nesprin-2 localization at the nuclear envelope. Expression of dominant negative Nesprin-2 constructs and knockdown studies in COS7 cells revealed that the presence of Nesprin-2 at the nuclear envelope is necessary for the proper localization of emerin. Our data imply a scaffolding function of Nesprin-2 at the nuclear membrane and suggest a potential involvement of this multi-isomeric protein in human disease.
Citation
Libotte, T., Zaim, H., Abraham, S., Padmakumar, V., Schneider, M., Lu, W., …Karakesisoglou, I. (2005). Lamin A/C-dependent localization of Nesprin-2, a giant scaffolder at the nuclear envelope. Molecular Biology of the Cell, 16(7), 3411-3424. https://doi.org/10.1091/mbc.e04-11-1009
Journal Article Type | Article |
---|---|
Publication Date | Jul 1, 2005 |
Deposit Date | Oct 1, 2008 |
Publicly Available Date | Oct 1, 2008 |
Journal | Molecular biology of the cell |
Publisher | American Society for Cell Biology |
Peer Reviewed | Peer Reviewed |
Volume | 16 |
Issue | 7 |
Pages | 3411-3424 |
DOI | https://doi.org/10.1091/mbc.e04-11-1009 |
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Copyright Statement
© 2005 by The American Society for Cell Biology
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