Mechanistic studies on acyl-transferase ribozymes and beyond

Hodgson, D.R.W.; Suga, H.

doi:10.1002/bip.10518

Mechanistic studies on acyl-transferase ribozymes and beyond

Hodgson, D.R.W.; Suga, H.

Authors

Professor David Hodgson d.r.w.hodgson@durham.ac.uk
Professor

H. Suga

Abstract

In vitro selection has allowed the isolation of many new ribozymes that are able to catalyze an ever-widening array of chemical transformations. Mechanistic studies on these selected ribozymes have provided valuable insight into the methods that RNA can invoke to overcome different catalytic tasks. We focus on the methods employed in these mechanistic studies using the acyl-transferase family of selected ribozymes as well-studied reference systems. Chemical and biochemical techniques have been used in tandem in order to draw conclusions on the various modes of catalysis employed by the different family members. In turn, this type of mechanistic information may provide a means for the redesign and optimization of existing ribozymes or the basis for new selection systems for more powerful RNA catalysts.

Citation

Hodgson, D., & Suga, H. (2004). Mechanistic studies on acyl-transferase ribozymes and beyond. Biopolymers, 73(1), 130-150. https://doi.org/10.1002/bip.10518

Journal Article Type	Article
Publication Date	Jan 1, 2004
Deposit Date	Jan 18, 2008
Journal	Biopolymers
Print ISSN	0006-3525
Electronic ISSN	1097-0282
Publisher	Wiley
Peer Reviewed	Peer Reviewed
Volume	73
Issue	1
Pages	130-150
DOI	https://doi.org/10.1002/bip.10518