Pullen, M. and Clark, N. and Zarinkamar, F. and Topping, J. and Lindsey, K. (2010) 'Analysis of vascular development in the hydra sterol biosynthetic mutants of Arabidopsis.', PLoS ONE., 5 (8). e12227.
Background: The control of vascular tissue development in plants is influenced by diverse hormonal signals, but their interactions during this process are not well understood. Wild-type sterol profiles are essential for growth, tissue patterning and signalling processes in plant development, and are required for regulated vascular patterning. Methodology/Principal Findings: Here we investigate the roles of sterols in vascular tissue development, through an analysis of the Arabidopsis mutants hydra1 and fackel/hydra2, which are defective in the enzymes sterol isomerase and sterol C-14 reductase respectively. We show that defective vascular patterning in the shoot is associated with ectopic cell divisions. Expression of the auxin-regulated AtHB8 homeobox gene is disrupted in mutant embryos and seedlings, associated with variably incomplete vascular strand formation and duplication of the longitudinal axis. Misexpression of the auxin reporter proIAA2:GUS and mislocalization of PIN proteins occurs in the mutants. Introduction of the ethylene-insensitive ein2 mutation partially rescues defective cell division, localization of PIN proteins, and vascular strand development. Conclusions: The results support a model in which sterols are required for correct auxin and ethylene crosstalk to regulate PIN localization, auxin distribution and AtHB8 expression, necessary for correct vascular development.
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|Publisher Web site:||http://dx.doi.org/10.1371/journal.pone.0012227|
|Publisher statement:||Copyright: © 2010 Pullen et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.|
|Date accepted:||No date available|
|Date deposited:||25 October 2011|
|Date of first online publication:||August 2010|
|Date first made open access:||No date available|
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