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A new chitinase-like xylanase inhibitor protein (XIP) from coffee (Coffea arabica) affects Soybean Asian rust (Phakopsora pachyrhizi) spore germination

Vasconcelos, E.A.; Santana, C.G.; Godoy, C.V.; Seixas, C.D.; Silva, M.S.; Moreira, L.R.S.; Oliviera-Neto, O.B.; Price, D.; Fitches, E.; Filho, E.D.F.; Mehta, A.; Gatehouse, J.A.; Grossi-de-Sa, M.F.

A new chitinase-like xylanase inhibitor protein (XIP) from coffee (Coffea arabica) affects Soybean Asian rust (Phakopsora pachyrhizi) spore germination Thumbnail


Authors

E.A. Vasconcelos

C.G. Santana

C.V. Godoy

C.D. Seixas

M.S. Silva

L.R.S. Moreira

O.B. Oliviera-Neto

D. Price

E.D.F. Filho

A. Mehta

J.A. Gatehouse

M.F. Grossi-de-Sa



Abstract

Background: Asian rust (Phakopsora pachyrhizi) is a common disease in Brazilian soybean fields and it is difficult to control. To identify a biochemical candidate with potential to combat this disease, a new chitinase-like xylanase inhibitor protein (XIP) from coffee (Coffea arabica) (CaclXIP) leaves was cloned into the pGAPZα-B vector for expression in Pichia pastoris. Results: A cDNA encoding a chitinase-like xylanase inhibitor protein (XIP) from coffee (Coffea arabica) (CaclXIP), was isolated from leaves. The amino acid sequence predicts a (β/α)8 topology common to Class III Chitinases (glycoside hydrolase family 18 proteins; GH18), and shares similarity with other GH18 members, although it lacks the glutamic acid residue essential for catalysis, which is replaced by glutamine. CaclXIP was expressed as a recombinant protein in Pichia pastoris. Enzymatic assay showed that purified recombinant CaclXIP had only residual chitinolytic activity. However, it inhibited xylanases from Acrophialophora nainiana by approx. 60% when present at 12:1 (w/w) enzyme:inhibitor ratio. Additionally, CaclXIP at 1.5 μg/μL inhibited the germination of spores of Phakopsora pachyrhizi by 45%. Conclusions: Our data suggests that CaclXIP belongs to a class of naturally inactive chitinases that have evolved to act in plant cell defence as xylanase inhibitors. Its role on inhibiting germination of fungal spores makes it an eligible candidate gene for the control of Asian rust.

Citation

Vasconcelos, E., Santana, C., Godoy, C., Seixas, C., Silva, M., Moreira, L., …Grossi-de-Sa, M. (2011). A new chitinase-like xylanase inhibitor protein (XIP) from coffee (Coffea arabica) affects Soybean Asian rust (Phakopsora pachyrhizi) spore germination. BMC Biotechnology, 11, Article 14. https://doi.org/10.1186/1472-6750-11-14

Journal Article Type Article
Publication Date Feb 1, 2011
Deposit Date Mar 29, 2012
Publicly Available Date Mar 29, 2024
Journal BMC Biotechnology
Publisher BioMed Central
Peer Reviewed Peer Reviewed
Volume 11
Article Number 14
DOI https://doi.org/10.1186/1472-6750-11-14

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Copyright Statement
© 2011 Vasconcelos et al; licensee BioMed Central Ltd.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.




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