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Cell-specific and lamin-dependent targeting of novel transmembrane proteins in the nuclear envelope

Malik, P; Korfali, N; Srsen, V; Lazou, V; Batrakou, DG; Zuleger, N; Kavanagh, DM; Wilkie, GS; Goldberg, MW; Schirmer, EC.

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Authors

P Malik

N Korfali

V Srsen

V Lazou

DG Batrakou

N Zuleger

DM Kavanagh

GS Wilkie

EC. Schirmer



Abstract

Nuclear envelope complexity is expanding with respect to identification of protein components. Here we test the validity of proteomics results that identified 67 novel predicted nuclear envelope transmembrane proteins (NETs) from liver by directly comparing 30 as tagged fusions using targeting assays. This confirmed 21 as NETs, but 4 only targeted in certain cell types, underscoring the complexity of interactions that tether NETs to the nuclear envelope. Four NETs accumulated at the nuclear rim in normal fibroblasts but not in fibroblasts lacking lamin A, suggesting involvement of lamin A in tethering them in the nucleus. However, intriguingly, for the NETs tested alternative mechanisms for nuclear envelope retention could be found in Jurkat cells that normally lack lamin A. This study expands by a factor of three the number of liver NETs analyzed, bringing the total confirmed to 31, and shows that several have multiple mechanisms for nuclear envelope retention.

Citation

Malik, P., Korfali, N., Srsen, V., Lazou, V., Batrakou, D., Zuleger, N., …Schirmer, E. (2010). Cell-specific and lamin-dependent targeting of novel transmembrane proteins in the nuclear envelope. Cellular and Molecular Life Sciences, 67(8), 1353-1369. https://doi.org/10.1007/s00018-010-0257-2

Journal Article Type Article
Publication Date Apr 1, 2010
Deposit Date Oct 11, 2010
Publicly Available Date Jun 12, 2012
Journal Cellular and Molecular Life Sciences
Print ISSN 1420-682X
Electronic ISSN 1420-9071
Publisher Springer
Peer Reviewed Peer Reviewed
Volume 67
Issue 8
Pages 1353-1369
DOI https://doi.org/10.1007/s00018-010-0257-2
Keywords Inner nuclear membrane, Outer nuclear membrane, Nuclear lamina, Integral membrane protein, Cell-type specificity.

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Copyright Statement
This article is published under a Creative Commons Attribution (CC-BY) license





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