Houck, Scott A. and Landsbury, Andrew and Clark, John I. and Quinlan, Roy A. (2011) 'Multiple sites in alpha B-crystallin modulate its interactions with desmin filaments assembled in vitro.', PLoS ONE., 6 (11). e25859.
Abstract
The β3- and β8-strands and C-terminal residues 155–165 of αB-crystallin were identified by pin arrays as interaction sites for various client proteins including the intermediate filament protein desmin. Here we present data using 5 well-characterised αB-crystallin protein constructs with substituted β3- and β8-strands and with the C-terminal residues 155–165 deleted to demonstrate the importance of these sequences to the interaction of αB-crystallin with desmin filaments. We used electron microscopy of negatively stained samples to visualize increased interactions followed by sedimentation assays to quantify our observations. A low-speed sedimentation assay measured the ability of αB-crystallin to prevent the self-association of desmin filaments. A high-speed sedimentation assay measured αB-crystallin cosedimentation with desmin filaments. Swapping the β8-strand of αB-crystallin or deleting residues 155–165 increased the cosedimentation of αB-crystallin with desmin filaments, but this coincided with increased filament-filament interactions. In contrast, substitution of the β3-strand with the equivalent αA-crystallin sequences improved the ability of αB-crystallin to prevent desmin filament-filament interactions with no significant change in its cosedimentation properties. These data suggest that all three sequences (β3-strand, β8-strand and C-terminal residues 155–165) contribute to the interaction of αB-crystallin with desmin filaments. The data also suggest that the cosedimentation of αB-crystallin with desmin filaments does not necessarily correlate with preventing desmin filament-filament interactions. This important observation is relevant not only to the formation of the protein aggregates that contain both desmin and αB-crystallin and typify desmin related myopathies, but also to the interaction of αB-crystallin with other filamentous protein polymers.
Item Type: | Article |
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Full text: | (VoR) Version of Record Available under License - Creative Commons Attribution. Download PDF (1146Kb) |
Status: | Peer-reviewed |
Publisher Web site: | https://doi.org/10.1371/journal.pone.0025859 |
Publisher statement: | Copyright: © 2011 Houck et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
Date accepted: | 13 September 2011 |
Date deposited: | 08 June 2012 |
Date of first online publication: | 09 November 2011 |
Date first made open access: | No date available |
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